| Title |
Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content
|
|---|---|
| Published in |
Nutrients, July 2018
|
| DOI | 10.3390/nu10070857 |
| Pubmed ID | |
| Authors |
Elisabeth Kurze, Vanessa Kock, Roberto Lo Scalzo, Klaus Olbricht, Wilfried Schwab |
| Abstract |
Birch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries (Fragaria × ananassa). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0.67 to 3.97 µg/g fresh weight. Mature fruits of red-, white- and yellow-fruited strawberry cultivars showed similar Fra a 1 concentrations. Compared to fresh strawberries, oven and solar-dried fruits contained slightly lower levels due to thermal treatment during processing. SDS-PAGE and Western blot analysis demonstrated degradation of recombinant Fra a 1.02 after prolonged (>10 min) thermal treatment at 99 °C. In conclusion, the genotype strongly determined the Fra a 1 quantity in strawberries and the color of the mature fruits does not relate to the amount of the PR10-protein. Cultivation conditions (organic and conventional farming) do not affect the Fra a 1 level, and seasonal effects were minor. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 15% |
| Researcher | 5 | 15% |
| Student > Master | 5 | 15% |
| Professor | 1 | 3% |
| Lecturer | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 15 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 18% |
| Biochemistry, Genetics and Molecular Biology | 5 | 15% |
| Medicine and Dentistry | 4 | 12% |
| Social Sciences | 1 | 3% |
| Chemistry | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 17 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 117. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 July 2018.
All research outputs
#305,121
of 23,094,276 outputs
Outputs from Nutrients
#746
of 17,753 outputs
Outputs of similar age
#7,485
of 327,941 outputs
Outputs of similar age from Nutrients
#17
of 377 outputs
Altmetric has tracked 23,094,276 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 17,753 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 23.4. This one has done particularly well, scoring higher than 95% of its peers.
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We're also able to compare this research output to 377 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.